| MWexperimental | 8 | kDa |
| MWexpected | 5 | kDa |
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log I(s)
1.10×10-2
1.10×10-3
1.10×10-4
1.10×10-5
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s, nm-1
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Structural Characterization of Plant Metallothionein
Dima Molodenskiy.SASDJH6 – The CxxC-linker-CxxC construct binding 1 Cd(II) ion in a CdCys4 site
A CxxC-linker-CxxC construct binding 1 Cd(II) ion in a CdCys4 site|
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Fits and models
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Rg, nm
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Synchrotron SAXS
data from solutions of
The CxxC-linker-CxxC construct binding 1 Cd(II) ion in a CdCys4 site
in
10 mM Tris, 50 mM NaCl, pH 7.4
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 6M detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 0.124 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 5.76 mg/ml was measured
at 20°C.
80 successive
0.100 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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