| MWexperimental | 372 | kDa |
| MWexpected | 366 | kDa |
| VPorod | 262 | nm3 |
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log I(s)
4.59×101
4.59×100
4.59×10-1
4.59×10-2
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s, nm-1
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Structural and biochemical characterization of RNA polymerase core and UvrD complex: a key component in transcription coupled DNA repair
Ravishankar Ramachandran.SASDJK4 – RNA polymerase core complex
DNA-directed RNA polymerase subunit alphaDNA-directed RNA polymerase subunit beta
DNA-directed RNA polymerase subunit beta'
DNA-directed RNA polymerase subunit omega
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Fits and models
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Synchrotron SAXS data from solutions of the RNA polymerase core complex in 10 mM Tris-Cl, 100 mM NaCl, 10 mM MgCl2, 0.1 mM EDTA, 5% Glycerol, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.0992 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.40 mg/ml was measured at 10°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Sample is likely aggregated. |
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