MWI(0) | 72 | kDa |
MWexpected | 83 | kDa |
VPorod | 93 | nm3 |
log I(s)
1.68×10-2
1.68×10-3
1.68×10-4
1.68×10-5
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s, nm-1
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X-ray synchrotron radiation scattering data from BcfH in 25 mM HEPES 150 mM NaCl, pH 7.5 were collected on the SAXS/WAXS beam line of the Australian Synchrotron (Melbourne, Australia) using a 2D Photon counting Pilatus 1M-W pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). Thirty five successive 1 second frames were collected at 0.3 mg/ml. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of the solvent-blank was subtracted. The models and corresponding fits include those derived from dummy-atom modelling using DAMMIN (16 models averaged and filtered using damaver), and CORAL (the best of 16 models) are provided here.
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