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Synchrotron SAXS data from solutions of YSD1_22 (Major Tail Protein) in 20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol, pH 7.8 were collected on the SAXS/WAXS beam line at the Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line co-flow size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 50.00 μl sample at 10 mg/ml was injected at a 0.40 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 22°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The major tail protein (YSD1-22) from flagellotropic bacteriophage YSD1 which infects Salmonella Typhi. Expressed in E. coli this protein is monomeric as it requires additional factors to polymerise into the bacteriophage tail structure. The ab initio models displayed in this entry are: i) Top, derived from the spatial alignment of an individual model cohort that has undergone bead-occupancy and volume-correction (DAMFILT model) and; ii) Bottom, a refined individual DAMMIN model and corresponding fit obtained from a spatially aligned model cohort consensus.
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s, nm-1
