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Synchrotron SAXS data from solutions of angiopoietin-like protein 4 in 50 mM Tris-HCl pH 7.4, 300 mM NaCl, 100 mM betaine, 500 mM arginine, pH 7.4 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus 100K detector at a sample-detector distance of 3.5 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 300.00 μl sample at 5 mg/ml was injected at a 0.80 ml/min flow rate onto a Wyatt WTC-030S5 5μm 300+, 7.8x300 mm column at 25°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Number of frames = UNKNOWN
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s, nm-1
