| MWexperimental | 86 | kDa |
| MWexpected | 85 | kDa |
| VPorod | 146 | nm3 |
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log I(s)
2.52×101
2.52×100
2.52×10-1
2.52×10-2
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s, nm-1
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Structural basis of client specificity in mitochondrial membrane-protein chaperones.
Sučec I, Wang Y, Dakhlaoui O, Weinhäupl K, Jores T, Costa D, Hessel A, Brennich M, Rapaport D, Lindorff-Larsen K, Bersch B, Schanda P, Sci Adv 6(51) (2020) Europe PMCSASDJP4 – Mitochondrial import inner membrane translocase subunits TIM9-TIM10 in complex with TIM23
Mitochondrial import inner membrane translocase subunit TIM9Mitochondrial import inner membrane translocase subunit TIM10
Mitochondrial import inner membrane translocase subunit TIM23
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Fits and models
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Synchrotron SAXS data from solutions of mitochondrial import inner membrane translocase subunits TIM9-TIM10 in complex with TIM23 in 50 mM Tris, 150 mM NaCl, pH 7.4 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 500.00 μl sample at 2 mg/ml was injected at a 0.20 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 25°C. 50 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Mw from Porod volume. |
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