| MWexperimental | 309 | kDa |
| MWexpected | 302 | kDa |
| VPorod | 950 | nm3 |
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log I(s)
1.01×105
1.01×104
1.01×103
1.01×102
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s, nm-1
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[Fe-S] cluster biogenesis and unusual assembly of the ISC scaffold complex in Plasmodium falciparum mitochondrion
Ravishankar Ramachandran.SASDJP7 – Cysteine desulfurase, Iron-sulfur cluster assembly protein, Protein ISD11 (IscS_IscU_Isd11) complex
Cysteine desulfurase, putativeIron-sulfur cluster assembly protein
Protein ISD11
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Fits and models
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SAXS data from solutions of the IscS_IscU_Isd11 complex in 50 mM Tris-Cl, 300 mM NaCl, 5% Glycerol, pH 7.5 were collected using an Anton Paar SAXSpace instrument at the CSIR-Central Drug Research Institute (Lucknow, India) equipped with a Dectris Mythen 2R1K detector at a sample-detector distance of 0.3 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 10°C. Two successive 30 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
CAUTION! The error on Rg (from Guinier) exceeds 1.5 nm. CAUTION! Porod volume is inconsistent with the quoted MW of 309 kDa. |
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