| MWI(0) | 38 | kDa |
| MWexpected | 39 | kDa |
| VPorod | 50 | nm3 |
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log I(s)
2.57×10-2
2.57×10-3
2.57×10-4
2.57×10-5
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s, nm-1
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The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection.
Askarian F, Uchiyama S, Masson H, Sørensen HV, Golten O, Bunæs AC, Mekasha S, Røhr ÅK, Kommedal E, Ludviksen JA, Arntzen MØ, Schmidt B, Zurich RH, van Sorge NM, Eijsink VGH, Krengel U, Mollnes TE, Lewis NE, Nizet V, Vaaje-Kolstad G, Nat Commun 12(1):1230 (2021) Europe PMCSASDJQ5 – Chitin-binding protein CbpD (Pseudomonas aeruginosa) at 24 °C
Chitin-binding protein CbpD|
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Fits and models
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SAXS data from solutions of CbpD in 15 mM Tris-HCl 150 mM NaCl, pH 7.5 were collected using a Bruker Nanostar instrument with an InCoatec Cu microsource (RECX, University of Oslo, Norway) equipped with a Våntec-2000 detector at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.18 mg/ml was measured at 24°C. Two successive 3600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
For the dummy-atom model representations: An individual DAMMIF model with the corresponding fit to the data is displayed along with a damfilt representation generated from the spatial alignment of nine individual models. |
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