The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection.

Askarian F, Uchiyama S, Masson H, Sørensen HV, Golten O, Bunæs AC, Mekasha S, Røhr ÅK, Kommedal E, Ludviksen JA, Arntzen MØ, Schmidt B, Zurich RH, van Sorge NM, Eijsink VGH, Krengel U, Mollnes TE, Lewis NE, Nizet V, Vaaje-Kolstad G, Nat Commun 12(1):1230 (2021) Europe PMC

SASDJR5 – Chitin-binding protein CbpD (Pseudomonas aeruginosa) at 37°C

Chitin-binding protein CbpD
MWexperimental 39 kDa
MWexpected 39 kDa
VPorod 63 nm3
log I(s) 6.44×10-2 6.44×10-3 6.44×10-4 6.44×10-5
Chitin-binding protein CbpD small angle scattering data  s, nm-1
ln I(s)
Chitin-binding protein CbpD Guinier plot ln 6.44×10-2 Rg: 3.5 nm 0 (3.5 nm)-2 s2
(sRg)2I(s)/I(0)
Chitin-binding protein CbpD Kratky plot 1.104 0 √3 sRg
Dmax: 15 nm

Data validation

There are no models related to this curve.

SAXS data from solutions of CbpD in 15 mM Tris-HCl 150 mM NaCl, pH 7.5 were collected using a Bruker Nanostar instrument with an InCoatec Cu microsource (RECX, University of Oslo, Norway) equipped with a Våntec-2000 detector at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.18 mg/ml was measured at 37°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

X-ray exposure time: UNKNOWN

Chitin-binding protein CbpD (CbpD)
Mol. type   Protein
Organism   Pseudomonas aeruginosa
Olig. state   Monomer
Mon. MW   39.2 kDa
 
UniProt   Q9I589 (26-389)
Sequence   FASTA