PfKsgA1 functions as a transcription initiation factor and interacts with the N-terminal region of the mitochondrial RNA polymerase of Plasmodium falciparum.

Gupta A, Shrivastava D, Shakya AK, Gupta K, Pratap JV, Habib S, Int J Parasitol (2020) Europe PMC

SASDJT2 – DNA-directed RNA polymerase (RPO41)

DNA-directed RNA polymerase
MWI(0) 150 kDa
MWexpected 150 kDa
VPorod 295 nm3
log I(s) 1.39×105 1.39×104 1.39×103 1.39×102
DNA-directed RNA polymerase small angle scattering data  s, nm-1
ln I(s)
DNA-directed RNA polymerase Guinier plot ln 1.40×105 Rg: 4.4 nm 0 (4.4 nm)-2 s2
(sRg)2I(s)/I(0)
DNA-directed RNA polymerase Kratky plot 1.104 0 √3 sRg
p(r)
DNA-directed RNA polymerase pair distance distribution function Rg: 4.1 nm 0 Dmax: 12.5 nm

Data validation

There are no models related to this curve.

SAXS data from solutions of RPO41 in 40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH 8 were collected using an Anton Paar SAXSpace instrument at the CSIR-Central Drug Research Institute (Lucknow, India) using a Dectris Mythen 2R1K detector at a sample-detector distance of 0.3 m and at a wavelength of λ = 0.148 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 10°C. Three successive 1800 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

DNA-directed RNA polymerase (RPO41)
Mol. type   Protein
Organism   Saccharomyces cerevisiae
Olig. state   Monomer
Mon. MW   149.7 kDa
 
UniProt   P13433 (30-1351)
Sequence   FASTA