SASDJT6 – Collagenase H C-terminal non-catalytic segments Polycystic Kidney disease domain 1, Polycystic Kidney disease domain 2 and Collagen binding domain (CBD) with Y689S, F712S, Y780S, H782S, Y796S and Y801S bound to collagen like-peptide

MWexperimental 44 kDa MWexpected 44 kDa VPorod 61 nm3 log I(s) 1.09×101 1.09×100 1.09×10-1 1.09×10-2  s, nm-1 Log plot Log-Log plot

ln I(s) ln 1.09×101 Rg: 4.0 nm 0 (4.0 nm)-2 s2 (sRg)2I(s)/I(0) 1.104 0 √3 sRg p(r) Rg: 4.6 nm 0 Dmax: 22 nm

Fits and models

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log I(s)  s, nm-1 +3 Δ/σ -3  s, nm-1 Fit validation Metric Value pcormap χ² 0 4.669 Worse Better

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Synchrotron SAXS data from solutions of Collagenase H C-terminal non-catalytic segments Polycystic Kidney disease domain 1, Polycystic Kidney disease domain 2 and Collagen binding domain (CBD) with Y689S, F712S, Y780S, H782S, Y796S and Y801S bound to collagen like-peptide in 50 mM HEPES, 100 mM NaCl, 5 mM CaCl2, pH 7.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 10°C. 33 successive 0.300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. Storage temperature = UNKNOWN

Collagen like-peptide [GPRG(POG)13] Mol. type   Protein Olig. state   Trimer Mon. MW   3.7 kDa Sequence   FASTA   Collagenase ColH (Polycystic kidney disease 1 (PKD1), Polycystic kidney disease domain 2 (PKD2) and Collagen binding domain (CBD) with Tyr689Ser, Phe712Ser, Tyr780Ser, His782Ser, Tyr796Ser and Tyr801) (ColH) Mol. type   Protein Organism   Hathewaya histolytica Olig. state   Monomer Mon. MW   33.2 kDa   UniProt   Q46085 (718-1021) Sequence   FASTA