| MWexperimental | 140 | kDa |
| MWexpected | 134 | kDa |
| VPorod | 240 | nm3 |
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log I(s)
4.28×101
4.28×100
4.28×10-1
4.28×10-2
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s, nm-1
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Visualizing functional dynamicity in the DNA-dependent protein kinase holoenzyme DNA-PK complex by integrating SAXS with cryo-EM.
Hammel M, Rosenberg DJ, Bierma J, Hura GL, Lees-Miller SP, Tainer JA, Prog Biophys Mol Biol (2020) Europe PMCSASDJV4 – X-ray repair cross-complementing proteins 5ΔCTR and 6
X-ray repair cross-complementing protein 6X-ray repair cross-complementing protein 5 ΔCTR
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Fits and models
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Synchrotron SAXS
data from solutions of
X-ray repair cross-complementing proteins 5ΔCTR and 6
in
50 mM Hepes, 50 mM KCl, 5 mM MgCl2, 5% glycerol and 0.2 mM DTT, pH 7.5
were collected
on the
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a Pilatus3 X 2M detector
at a sample-detector distance of 2.1 m and
at a wavelength of λ = 0.1127 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 55.00 μl sample
at 5 mg/ml was injected at a 0.50 ml/min flow rate
onto a Shodex KW-800 series column
at 20°C.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Storage temperature = UNKNOWN. Number of frames = UNKNOWN |
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