| MWexperimental | 31 | kDa |
| MWexpected | 37 | kDa |
| VPorod | 41 | nm3 |
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log I(s)
4.82×10-3
4.82×10-4
4.82×10-5
4.82×10-6
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s, nm-1
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Steric and Electronic Interactions at Gln154 in ZEITLUPE Induce Reorganization of the LOV Domain Dimer Interface.
Pudasaini A, Green R, Song YH, Blumenfeld A, Karki N, Imaizumi T, Zoltowski BD, Biochemistry (2020) Europe PMCSASDJX5 – Solution Scattering of Zeitlupe G46S:G80R
Adagio protein 1 (Zeitlupe G46S:G80R)|
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Fits and models
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Synchrotron SAXS data from solutions of Zeitlupe (G46S:G80R) in 50 mM HEPES, 100 mM NaCl, 2 mM TCEP, pH 8 were collected on the G1 beam line at the Cornell High Energy Synchrotron Source (CHESS, Ithaca, NY, USA) using a Pilatus 100K detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.12511 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample at 20 mg/ml was injected at a 0.15 ml/min flow rate onto a GE Superdex 200 Increase 5/150 column at 4°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Total X-ray exposure time: UNKNOWN. |
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