| MWexperimental | 480 | kDa |
| MWexpected | 468 | kDa |
| VPorod | 918 | nm3 |
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log I(s)
5.55×102
5.55×101
5.55×100
5.55×10-1
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s, nm-1
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Visualizing functional dynamicity in the DNA-dependent protein kinase holoenzyme DNA-PK complex by integrating SAXS with cryo-EM.
Hammel M, Rosenberg DJ, Bierma J, Hura GL, Lees-Miller SP, Tainer JA, Prog Biophys Mol Biol (2020) Europe PMCSASDJY4 – Autophosphorylated DNA-dependent protein kinase (DNA-PKcs)
DNA-dependent protein kinase catalytic subunit|
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Fits and models
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Synchrotron SAXS
data from solutions of
Autophosphorylated DNA-dependent protein kinase (DNA-PKcs)
in
50 mM Tris-HCl, 100 mM KCl, 5% (v/v) glycerol, 0.2 mM EDTA containing 0.1 mM benzamidine, 0.2 mM PMSF and 0.2 µg/ml pepstatin, pH 8
were collected
on the
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a MAR 165 CCD detector
at a sample-detector distance of 1.5 m and
at a wavelength of λ = 0.103 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1.5 and 15 mg/ml were measured
at 20°C.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Storage temperature = UNKNOWN. Number of frames = UNKNOWN |
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