| MWexperimental | 86 | kDa |
| MWexpected | 70 | kDa |
| VPorod | 127 | nm3 |
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log I(s)
2.30×10-2
2.30×10-3
2.30×10-4
2.30×10-5
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s, nm-1
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Low-resolution structures of modular nanotransporters shed light on their functional activity
Khramtsov Y, Vlasova A, Vlasov A, Rosenkranz A, Ulasov A, Ryzhykau Y, Kuklin A, Orekhov A, Eydlin I, Georgiev G, Gordeliy V, Sobolev A, Acta Crystallographica Section D Structural Biology 76(12):1270-1279 (2020) DOISASDJY7 – A modular diphtheria toxin/flavohemoprotein nanotransporter (MNT) fused to a melanocyte stimulating hormone ligand module (MNT-MSH)
Modular nanotransporter with a melanocyte stimulating hormone ligand module|
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Fits and models
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Rg, nm
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Synchrotron SAXS data from solutions of MNT-MSH in 150 mM NaCl, 10 mM phosphate buffer saline, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09918 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 500.00 μl sample at 4.2 mg/ml was injected at a 0.75 ml/min flow rate onto a GE Superdex 200 Increase 10/300 column at 20°C. 10 successive 1 second frames were collected through the SEC elution peak of the sample. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The first part of the amino acid sequence of MNT-MSH encompasses sequences homologous to Diphtheria toxin (https://www.uniprot.org/uniprot/P00587) followed by Flavohemoprotein (https://www.uniprot.org/uniprot/P24232). The C-terminal region has a sequence homology to the Large T antigen from Simian virus 40 (SV40) (https://www.uniprot.org/uniprot/P03070). |
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