| MWI(0) | 39 | kDa |
| MWexpected | 39 | kDa |
| VPorod | 64131 | nm3 |
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log I(s)
3.08×10-2
3.08×10-3
3.08×10-4
3.08×10-5
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s, nm-1
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The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection.
Askarian F, Uchiyama S, Masson H, Sørensen HV, Golten O, Bunæs AC, Mekasha S, Røhr ÅK, Kommedal E, Ludviksen JA, Arntzen MØ, Schmidt B, Zurich RH, van Sorge NM, Eijsink VGH, Krengel U, Mollnes TE, Lewis NE, Nizet V, Vaaje-Kolstad G, Nat Commun 12(1):1230 (2021) Europe PMCSASDK42 – Chitin-binding protein CbpD (Pseudomonas aeruginosa), ESRF BM29 data
Chitin-binding protein CbpD|
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Fits and models
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Synchrotron SAXS
data from solutions of
Chitin-binding protein CbpD (Pseudomonas aeruginosa), ESRF BM29 data
in
15 mM Tris-HCl 150 mM NaCl, pH 7.5
were collected
on the
BM29 beam line
at the ESRF storage ring
(Grenoble, France)
using a Pilatus 1M detector
at a sample-detector distance of 2.8 m and
at a wavelength of λ = 0.991862 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 0.3 and 2.5 mg/ml were measured
at 20°C.
10 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
CbpD was measured at four concentration: 2.50, 1.25, 0.63, 0.31 mg/mL at 20 °C at ESRF BM29. Data extrapolated to infinite dilution and scaled to 1 mg/mL. |
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