Structural insights into kinetoplastid coronin oligomerization domain and F-actin interaction

Parihar P, Singh A, Karade S, Sahasrabuddhe A, Pratap J, Current Research in Structural Biology (2021) DOI

SASDK46 – Coronin from Trypanosoma brucei brucei (C-terminal coiled coil domain)

Coronin
MWI(0) 32 kDa
MWexpected 21 kDa
VPorod 22 nm3
log I(s) 5.96×103 5.96×102 5.96×101 5.96×100
Coronin small angle scattering data  s, nm-1
ln I(s)
Coronin Guinier plot ln 5.96×103 Rg: 3.3 nm 0 (3.3 nm)-2 s2
(sRg)2I(s)/I(0)
Coronin Kratky plot 1.104 0 √3 sRg
p(r)
Coronin pair distance distribution function Rg: 3.3 nm 0 Dmax: 12.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Coronin GASBOR model
Coronin GASBOR model
SAXS data from solutions of the C-terminal coiled coil domain of coronin in 100 mM Tris-HCl, 100 mM NaCl, pH 7.4 were collected using an Anton Paar SAXSpace instrument (CSIR - Institute of Microbial Technology (IMTech), India) equipped with a Mythen 1K detector at a sample-detector distance of 0.3 m and at a wavelength of λ = 0.148 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 10°C. Two successive 120 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Coronin (coronin coiled coil)
Mol. type   Protein
Organism   Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Olig. state   Tetramer
Mon. MW   5.2 kDa
 
UniProt   Q57W63 (476-523)
Sequence   FASTA