| MWexperimental | 240 | kDa |
| MWexpected | 169 | kDa |
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log I(s)
1.68×100
1.68×10-1
1.68×10-2
1.68×10-3
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s, nm-1
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Molecular model of a sensor of two-component signaling system
Ryzhykau Y, Orekhov P, Rulev M, Vlasov A, Melnikov I, Volkov D, Nikolaev M, Zabelskii D, Murugova T, Chupin V, Rogachev A, Gruzinov A, Svergun D, Brennich M, Gushchin I, Soler-Lopez M, Bothe A, Büldt G, Leonard G, Engelhard M, Kuklin A, Gordeliy V, Scientific Reports 11(1) (2021) DOISASDK47 – SANS data for the sensory rhodopsin II / transducer complex in detergent at 1.4 M NaCl
Sensory rhodopsin II from Natronbacterium pharaonisSensory rhodopsin II transducer from Natronomonas pharaonis
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Fits and models
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SANS data from solutions of the sensory rhodopsin II / transducer complex in 1400 mM NaCl, 49.4 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pD 8 were collected using the YuMO (IBR-2) instrument (FLNP, JINR; Dubna, Russian Federation) equipped with a He3-fulfilled, 8 independent wire detector. One solute concentration of 0.33 mg/ml was measured at 20°C. SANS measurements were performed with a two-detector system (sample-detector distances of 4.5 m and 12.97 m). The wavelengths used are from 0.05 to 0.8 nm, the contributions of which are separated using time-of-flight technology.
The protein of study is a non-fused complex of sensory rhodopsin II (NpSRII, UniProt ID P42196) with its cognate transducer (NpHtrII, UniProt ID P42259) from Nartonomonas pharaonis. The NpSRII/NpHtrII complex in 0.05% (w/v) n-Dodecyl β-D-maltoside (DDM) detergent forms dimers and trimers of dimers at 2800 mM NaCl, the volume fractions of which are 81.7% and 18.3%, respectively. |
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