Proteolysis and multimerization regulate signaling along the two-component regulatory system AdeRS.

Ouyang Z, Zheng F, Zhu L, Felix J, Wu D, Wu K, Gutsche I, Wu Y, Hwang PM, She J, Wen Y, iScience 24(5):102476 (2021) Europe PMC

SASDK48 – Histidine kinase AdeS - DHp-CA Domain

Histidine kinase
MWexperimental 54 kDa
MWexpected 51 kDa
VPorod 85 nm3
log I(s) 6.69×101 6.69×100 6.69×10-1 6.69×10-2
Histidine kinase small angle scattering data  s, nm-1
ln I(s)
Histidine kinase Guinier plot ln 6.69×101 Rg: 2.8 nm 0 (2.8 nm)-2 s2
(sRg)2I(s)/I(0)
Histidine kinase Kratky plot 1.104 0 √3 sRg
p(r)
Histidine kinase pair distance distribution function Rg: 2.8 nm 0 Dmax: 9.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Histidine kinase MULTIFOXS model
Synchrotron SAXS data from solutions of the DHp-CA domain of histidine kinase AdeS in 20 mM Tris, 150 mM NaCl, pH 7.4 were collected on the BL19U2 beam line at the Shanghai Synchrotron Radiation Facility (SSRF; Shanghai, China) using a Pilatus 1M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 24°C. 20 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Histidine kinase (AdeS)
Mol. type   Protein
Organism   Acinetobacter baumannii
Olig. state   Dimer
Mon. MW   25.7 kDa
 
UniProt   Q93E22 (130-357)
Sequence   FASTA
 
PDB ID   7CCH