Synchrotron SAXS data from solutions of the Gemin2/delta linker Survival Motor Neuron-like protein 1 (SMN1, Δ36-119) complex from Schizosaccharomyces pombe in 150 mM NaCl, 20 mM HEPES, 1 mM DTT, pH 7.5 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample at 13.3 mg/ml was injected at a 1.00 ml/min flow rate onto a GE Superdex 200 10/300 column at 20°C. 1800 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
For this entry, SEC-SAXS frames 806-825 were scaled and averaged and background subtracted using scaled and averaged buffer frames 306-485. Peak concentration of the chromatogram determined from UV280 trace was at 2.2 g/L (~84 µM).
s, nm-1
