Conformational landscape of multidomain SMAD proteins

Gomes T, Martin-Malpartida P, Ruiz L, Aragón E, Cordeiro T, Macias M, Computational and Structural Biotechnology Journal (2021) DOI

SASDK99 – Mothers against decapentaplegic homolog 2, SMAD2, C-terminus deletion (Δ461-467) 1 mg/ml

Mothers against decapentaplegic homolog 2 (C-terminus deletion)
MWexperimental 33 kDa
MWexpected 158 kDa
log I(s) 1.12×101 1.12×100 1.12×10-1 1.12×10-2
Mothers against decapentaplegic homolog 2 (C-terminus deletion) small angle scattering data  s, nm-1
ln I(s)
Mothers against decapentaplegic homolog 2 (C-terminus deletion) Guinier plot ln 1.12×101 Rg: 4.1 nm 0 (4.1 nm)-2 s2
(sRg)2I(s)/I(0)
Mothers against decapentaplegic homolog 2 (C-terminus deletion) Kratky plot 1.104 0 3 sRg
Dmax: 17 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Mothers against decapentaplegic homolog 2, SMAD2, C-terminus deletion (Δ461-467) 1 mg/ml in 20 mM Tris, 150 mM NaCl, pH 7.2 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Protein in a concentration-dependent monomer-dimer-timer equilibrium. X-ray wavelength: UNKNOWN. X-ray exposure time: UNKNOWN. Sample detector distance: UNKNOWN.

Mothers against decapentaplegic homolog 2 (C-terminus deletion) (SMAD2(Δ461-467))
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Other
Mon. MW   52.6 kDa
 
UniProt   Q15796 (1-460)
Sequence   FASTA