Mechanistic insights into RNA surveillance by the canonical poly(A) polymerase Pla1 of the MTREC complex.

Soni K, Sivadas A, Horvath A, Dobrev N, Hayashi R, Kiss L, Simon B, Wild K, Sinning I, Fischer T, Nat Commun 14(1):772 (2023) Europe PMC

SASDKD6 – NURS complex subunit red1

NURS complex subunit red1
MWexperimental 13 kDa
MWexpected 8 kDa
VPorod 12 nm3
log I(s) 2.48×104 2.48×103 2.48×102 2.48×101
NURS complex subunit red1 small angle scattering data  s, nm-1
ln I(s)
NURS complex subunit red1 Guinier plot ln 2.49×104 Rg: 2.5 nm 0 (2.5 nm)-2 s2
(sRg)2I(s)/I(0)
NURS complex subunit red1 Kratky plot 1.104 0 3 sRg
p(r)
NURS complex subunit red1 pair distance distribution function Rg: 2.5 nm 0 Dmax: 8.7 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Red1 in 20 mM HEPES, pH 7.5, 150 mM NaCl, 1mM DTT, were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124193 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.00 mg/ml was measured at 20°C. 40 successive 0.195 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tags: idp
NURS complex subunit red1 (Red1)
Mol. type   Protein
Organism   Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Olig. state   Monomer
Mon. MW   7.6 kDa
 
UniProt   Q9UTR8 (288-345)
Sequence   FASTA