| MWexperimental | 42 | kDa |
| MWexpected | 52 | kDa |
| VPorod | 45 | nm3 |
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log I(s)
2.72×10-2
2.72×10-3
2.72×10-4
2.72×10-5
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s, nm-1
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The DNA binding domain of the Vibrio vulnificus SmcR transcription factor is flexible and binds diverse DNA sequences.
Newman JD, Russell MM, Fan L, Wang YX, Gonzalez-Gutierrez G, van Kessel JC, Nucleic Acids Res 49(10):5967-5984 (2021) Europe PMCSASDKE8 – LuxR family transcriptional regulator, SmcR N55I mutant
LuxR family transcriptional regulator, N55I|
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Fits and models
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Synchrotron SAXS data from solutions of the LuxR family transcriptional regulator, SmcR N55I mutant, in 200 mM NaCl, 25 mM Tris, 1 mM DTT, 0.5 mM EDTA, pH 7.5 were collected on the 12ID-B SAXS/WAXS beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.09322 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 20°C. 45 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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