The solution structures of higher-order human telomere G-quadruplex multimers.

Monsen RC, Chakravarthy S, Dean WL, Chaires JB, Trent JO, Nucleic Acids Res (2021) Europe PMC

SASDKF3 – Human telomere monomer G4 hybrid-2 form (2JSL)

Human telomere G-quadruplex hybrid-2 form
MWexperimental 7 kDa
MWexpected 8 kDa
VPorod 9 nm3
log I(s) 9.56×10-3 9.56×10-4 9.56×10-5 9.56×10-6
Human telomere G-quadruplex hybrid-2 form small angle scattering data  s, nm-1
ln I(s)
Human telomere G-quadruplex hybrid-2 form Guinier plot ln 9.56×10-3 Rg: 1.2 nm 0 (1.2 nm)-2 s2
(sRg)2I(s)/I(0)
Human telomere G-quadruplex hybrid-2 form Kratky plot 1.104 0 √3 sRg
p(r)
Human telomere G-quadruplex hybrid-2 form pair distance distribution function Rg: 1.2 nm 0 Dmax: 3.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Human telomere G-quadruplex hybrid-2 form DAMMIN model
Synchrotron SAXS data from solutions of Human telomere monomer G4 hybrid-2 form (2JSL) in 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH 7.2 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory storage ring (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.5 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 300.00 μl sample at 7 mg/ml was injected at a 0.75 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 20°C. Four successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Human telomere G-quadruplex hybrid-2 form (2JSL)
Mol. type   DNA
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   8.0 kDa
Sequence   FASTA