| MWexperimental | 64 | kDa |
| MWexpected | 58 | kDa |
| VPorod | 99 | nm3 |
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log I(s)
1.08×10-2
1.08×10-3
1.08×10-4
1.08×10-5
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s, nm-1
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High Conformational Flexibility of the E2F1/DP1/DNA Complex.
Saad D, Paissoni C, Chaves-Sanjuan A, Nardini M, Mantovani R, Gnesutta N, Camilloni C, J Mol Biol 433(18):167119 (2021) Europe PMCSASDKH2 – E2F1/DP1 in complex with a 15-mer DNA oligo
Transcription factor E2F115-mer DNA oligo of the human E2F1 promoter
E2F dimerization partner 1
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Fits and models
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Synchrotron SAXS
data from solutions of
E2F1/DP1 in complex with a 15-mer DNA oligo
in
50 mM Tris-Cl pH 8.0, 150 mM NaCl and 2 mM DTT, pH 8
were collected
on the
B21 beam line
at the Diamond Light Source storage ring
(Didcot, UK)
using a Pilatus 2M detector
at a sample-detector distance of 3.7 m and
at a wavelength of λ = 0.0946 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample
at 4 mg/ml was injected at a 0.08 ml/min flow rate
onto a GE Superdex 200 Increase 3.2/300 column
at 20°C.
20 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Storage temperature = UNKNOWN |
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