The solution structures of higher-order human telomere G-quadruplex multimers.

Monsen RC, Chakravarthy S, Dean WL, Chaires JB, Trent JO, Nucleic Acids Res (2021) Europe PMC

SASDKJ3 – Human telomere DNA d(TTAGGG)96

Human Telomere 96mer
MWexperimental 31 kDa
MWexpected 31 kDa
VPorod 38 nm3
log I(s) 2.02×10-2 2.02×10-3 2.02×10-4 2.02×10-5
Human Telomere 96mer small angle scattering data  s, nm-1
ln I(s)
Human Telomere 96mer Guinier plot ln 2.02×10-2 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Human Telomere 96mer Kratky plot 1.104 0 √3 sRg
p(r)
Human Telomere 96mer pair distance distribution function Rg: 3.3 nm 0 Dmax: 10.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Human Telomere 96mer DAMMIF model
Synchrotron SAXS data from solutions of Human telomere DNA d(TTAGGG)96 in 6 mM Na2HPO4, 2 mM NaH2PO4, 1 mM Na2EDTA, 185 mM KCl, pH 7.2 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory storage ring (Lemont, IL, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 3.5 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 440.00 μl sample at 6 mg/ml was injected at a 0.70 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 20°C. Four successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Human Telomere 96mer (Tel96)
Mol. type   DNA
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   30.6 kDa
Sequence   FASTA