Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes

Krichel B, Bylapudi G, Schmidt C, Blanchet C, Schubert R, Brings L, Koehler M, Zenobi R, Svergun D, Lorenzen K, Madhugiri R, Ziebuhr J, Uetrecht C, Science Advances 7(10):eabf1004 (2021) DOI

SASDKJ4 – SARS-CoV-2 nsp7 and nsp8

Replicase polyprotein 1a - nsp7
Replicase polyprotein 1a - nsp8
MWexperimental 56 kDa
MWexpected 62 kDa
VPorod 92 nm3
log I(s) 4.45×102 4.45×101 4.45×100 4.45×10-1
Replicase polyprotein 1a - nsp7 Replicase polyprotein 1a - nsp8 small angle scattering data  s, nm-1
ln I(s)
Replicase polyprotein 1a - nsp7 Replicase polyprotein 1a - nsp8 Guinier plot ln 4.45×102 Rg: 3.4 nm 0 (3.4 nm)-2 s2
(sRg)2I(s)/I(0)
Replicase polyprotein 1a - nsp7 Replicase polyprotein 1a - nsp8 Kratky plot 1.104 0 √3 sRg
Dmax: 13.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Replicase polyprotein 1a - nsp7 Replicase polyprotein 1a - nsp8 PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of the SARS-CoV-2 nsp7/nsp8 complex in 50 mM Tris, pH 8.0, 100 mM NaCl, 4 mM DTT, 4 mM MgCl2, pH 8 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). The data displayed for this entry shows the scattering from a sample at 2.08 mg/ml measured at 20°C. Concentration series SAXS data spanning 1.23 - 32.1 mg/ml are provided in the full-entry zip archive. 20 successive 0.0950 second frames were collected per sample and corresponding solvent blank. All data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Replicase polyprotein 1a - nsp7 (nsp7)
Mol. type   Protein
Organism   Severe acute respiratory syndrome coronavirus 2
Olig. state   Other
Mon. MW   9.2 kDa
 
UniProt   P0DTC1 (3860-3942)
Sequence   FASTA
 
Replicase polyprotein 1a - nsp8 (nsp8)
Mol. type   Protein
Organism   Severe acute respiratory syndrome coronavirus 2
Olig. state   Other
Mon. MW   21.9 kDa
 
UniProt   P0DTC1 (3943-4140)
Sequence   FASTA
 
PDB ID   2AHM