The Central Region of Testican-2 Forms a Compact Core and Promotes Cell Migration

Krajnc A, Gaber A, Lenarčič B, Pavšič M, International Journal of Molecular Sciences 21(24):9413 (2020) DOI

SASDKK2 – Testican-2 (23-379) Ca-loaded

Testican-2
MWexperimental 40 kDa
MWexpected 40 kDa
VPorod 78 nm3
log I(s) 2.26×104 2.26×103 2.26×102 2.26×101
Testican-2 small angle scattering data  s, nm-1
ln I(s)
Testican-2 Guinier plot ln 2.27×104 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Testican-2 Kratky plot 1.104 0 √3 sRg
p(r)
Testican-2 pair distance distribution function Rg: 3.3 nm 0 Dmax: 12.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Testican-2 (23-379) Ca-loaded Rg histogram Rg, nm
Testican-2 EOM/RANCH model
Testican-2 EOM/RANCH model
Testican-2 EOM/RANCH model
Testican-2 EOM/RANCH model
log I(s)
 s, nm-1
Testican-2 DAMMIN model
Synchrotron SAXS data from solutions of human testican-2 (residues 23-379) in 20 mM Na-HEPES, 130 mM NaCl, 3 mM CaCl2, 5 % (v/v) glycerol pH 7.4, was collected at the EMBL P12 beamline of the Petra III (DESY, Hamburg, Germany) using Pilatus 2M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle), at room temperature (20°C). The data was normalized to the intensity of the transmitted beam and radially averaged, and scattering of the solvent-blank was substracted. Data was extrapolated to zero concentration.

Number of frames = UNKNOWN

Testican-2 (T2d)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   40.0 kDa
 
UniProt   Q92563 (23-379)
Sequence   FASTA