| MWexperimental | 33 | kDa |
| MWexpected | 33 | kDa |
| VPorod | 54 | nm3 |
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log I(s)
1.64×10-2
1.64×10-3
1.64×10-4
1.64×10-5
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s, nm-1
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The structure and flexibility analysis of the Arabidopsis synaptotagmin 1 reveal the basis of its regulation at membrane contact sites
Benavente J, Siliqi D, Infantes L, Lagartera L, Mills A, Gago F, Ruiz-López N, Botella M, Sánchez-Barrena M, Albert A, Life Science Alliance 4(10):e202101152 (2021) DOISASDKK9 – C2AB construct of synaptotagmin-1 (SYT1) in presence of Ca cations
Synaptotagmin-1|
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Fits and models
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SAXS data were collected in the bioSAXS beamline B21, at Diamond Light Source, Harwell, United Kingdom. For the SYT1C2AB, 45 μl of sample with a concentration of 10 mg/ml, were injected onto a Superdex 200 increase 3.2/300 column at 20°C using 50 mM Tris, pH8, 50 mM NaCl as the running buffer. The output flow from the Agilent HPLC was directed through a 1.6 mm diameter quartz capillary cell held in vacuum. The flow rate was set to 0.08 mL min-1 and 1162 frames (with an exposure time of 3 s) were collected using a PILATUS 2M (Dectris, Switzerland) detector at the distance of 3.7 m from the sample. The collected two-dimensional images were corrected for variations in beam current, normalized for exposure time and processed into one- dimensional scattering curves using GDA and the DAWN software (Diamond Light Source, UK). The background was manually subtracted using the program ScÅtter and CHROMIXS (ATSAS).
The final concentration of Ca2+ cations is 1 mM. |
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