| MWexperimental | 57 | kDa |
| MWexpected | 62 | kDa |
| VPorod | 88 | nm3 |
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log I(s)
7.79×10-2
7.79×10-3
7.79×10-4
7.79×10-5
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s, nm-1
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Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA
Garcia-Rodriguez G, Charlier D, Wilmaerts D, Michiels J, Loris R, Nucleic Acids Research 49(12):7164-7178 (2021) DOISASDKL2 – NRD-HEPN - mRNA endoribonuclease toxin LS from Escherichia coli (strain K12)
NRD-HEPN truncated variant of RnlA endoribonuclease|
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Fits and models
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Synchrotron SAXS
data from solutions of
NRD-HEPN - mRNA endoribonuclease toxin LS from Escherichia coli (strain K12)
in
20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH 8
were collected
on the
SWING beam line
at the SOLEIL storage ring
(Saint-Aubin, France)
using a Eiger 4M detector
at a wavelength of λ = 1.033 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 60.00 μl sample
at 24 mg/ml was injected at a 0.20 ml/min flow rate
onto a Agilent Bio SEC-3, 300 Å column
at 16°C.
480 successive
0.990 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The sample in the previously defined buffer (after Ni-NTA and gel filtration purification) was flash-frozen in (l)N2. Once at the beamline the sample was quickly thawed, spun down and concentration checked before being applied onto an HPLC column connected to a quartz capillary for measurement. |
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