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Synchrotron SAXS data from solutions of carboxypeptidase-related protein (S10drt1) in 20 mM Tris-HCl, 200 mM NaCl, pH 8 were collected on the BL-18 beam line at INDUS-2 (Indore, India) using a MAR 345 Image Plate detector at a sample-detector distance of 3.2 m and at a wavelength of λ = 0.07749 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.00 mg/ml was measured at 37°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The reported experimental molecular weight was deduced by the approach (SAXS MoW2) described in, Piiadov V, Ares de Araújo E, Oliveira Neto M, Craievich AF, Polikarpov I. SAXSMoW 2.0: Online calculator of the molecular weight of proteins in dilute solution from experimental SAXS data measured on a relative scale. Protein Sci. 2019 Feb;28(2):454-463. doi: 10.1002/pro.3528. Epub 2018 Dec 13. PMID: 30371978; PMCID: PMC6319763. X-ray exposure time: UNKNOWN. The errors on the scattering intensities are severely overestimated.
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Carboxypeptidase-related protein
(S10Drt1)
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| Mol. type |
|
Protein |
| Organism |
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Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) |
| Olig. state |
|
16-mer |
| Mon. MW |
|
56.5 kDa |
| |
| UniProt |
|
Q9RVQ8
(27-500)
|
| Sequence |
|
FASTA |
| |
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s, nm-1
