Wide-Angle X-ray Scattering (WAXS) Analysis of Small Nucleic Acids

Yen-Lin Chen.

SASDKM5 – Metastasis-Associated Lung Adenocarcinoma Transcript 1

Metastasis-Associated Lung Adenocarcinoma Transcript 1
MWexperimental 23 kDa
MWexpected 23 kDa
log I(s) 3.02×101 3.02×100 3.02×10-1 3.02×10-2
Metastasis-Associated Lung Adenocarcinoma Transcript 1 small angle scattering data  s, nm-1
ln I(s)
Metastasis-Associated Lung Adenocarcinoma Transcript 1 Guinier plot ln 3.03×101 Rg: 2.3 nm 0 (2.3 nm)-2 s2
(sRg)2I(s)/I(0)
Metastasis-Associated Lung Adenocarcinoma Transcript 1 Kratky plot 1.104 0 3 sRg
p(r)
Metastasis-Associated Lung Adenocarcinoma Transcript 1 pair distance distribution function Rg: 2.4 nm 0 Dmax: 7.9 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Metastasis-Associated Lung Adenocarcinoma Transcript 1 in 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 μM EDTA, pH 7 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA) using a Pilatus3 S 1M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.092 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.33 mg/ml was measured at 10°C. 15 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Metastasis-Associated Lung Adenocarcinoma Transcript 1 (MALAT1)
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   23.4 kDa
Sequence   FASTA