NADPH-dependent sulfite reductase flavoprotein adopts an extended conformation unique to this diflavin reductase

Tavolieri A, Murray D, Askenasy I, Pennington J, McGarry L, Stanley C, Stroupe M, Journal of Structural Biology 205(2):170-179 (2019) DOI

SASDKN7 – Sulfite reductase flavoprotein-60-ΔAAPSQS

Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein)

MW^experimental	60	kDa
MW^expected	61	kDa
V^Porod	73	nm³

log I(s) 2.63×10^-1 2.63×10^-2 2.63×10^-3 2.63×10^-4

Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) Guinier plot

ln 2.64×10^-1 R_g: 3.2 nm 0 (3.2 nm)^-2 s²

(sR_g)²I(s)/I(0)

Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 3.3 nm 0 D_max: 11.3 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.4

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.039
1.775

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

SANS data from solutions of Sulfite reductase flavoprotein-60-ΔAAPSQS in 50 mM KPi, 100 mM NaCl, 1 mM EDTA, pH 7.8 were collected using the EQ-SANS (BL-6) beam line (Spallation Neutron Source, Oak Ridge National Laboratory, USA) equipped with a GE linear position-sensitive detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.25 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 8°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN

Sulfite reductase [NADPH] flavoprotein alpha-component (Assimilatory NADPH-dependent sulfite reductase flavoprotein) (SiRFP-60)
Mol. type		Protein
Organism		Escherichia coli (strain K12)
Olig. state		Monomer
Mon. MW		60.7 kDa

UniProt		P38038 (53-599)
Sequence		FASTA