Mycobacterium tuberculosis Rv2324 is a multifunctional feast/famine regulatory protein involved in growth, DNA replication and damage control.

Dubey S, Maurya RK, Shree S, Kumar S, Jahan F, Krishnan MY, Ramachandran R, Int J Biol Macromol 252:126459 (2023) Europe PMC

SASDKQ7 – Probable transcriptional regulatory protein Lrp/AsnC protein Rv2324 in the presence of methionine

Probable transcriptional regulatory protein (Probably AsnC-family)
MWexperimental 117 kDa
MWexpected 143 kDa
VPorod 246 nm3
log I(s) 1.91×105 1.91×104 1.91×103 1.91×102
Probable transcriptional regulatory protein (Probably AsnC-family) small angle scattering data  s, nm-1
ln I(s)
Probable transcriptional regulatory protein (Probably AsnC-family) Guinier plot ln 1.92×105 Rg: 4.0 nm 0 (4.0 nm)-2 s2
(sRg)2I(s)/I(0)
Probable transcriptional regulatory protein (Probably AsnC-family) Kratky plot 1.104 0 √3 sRg
p(r)
Probable transcriptional regulatory protein (Probably AsnC-family) pair distance distribution function Rg: 4.3 nm 0 Dmax: 13.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Probable transcriptional regulatory protein (Probably AsnC-family) DAMMIF model
Probable transcriptional regulatory protein (Probably AsnC-family) DAMFILT model
log I(s)
 s, nm-1
Probable transcriptional regulatory protein (Probably AsnC-family) CHIMERA model
log I(s)
 s, nm-1
Probable transcriptional regulatory protein (Probably AsnC-family) GASBOR model
SAXS data from solutions of the probable transcriptional regulatory protein Lrp/AsnC protein Rv2324 in 20 mM Tris, 150 mM NaCl, 2 mM EDTA, 2 mM methionine, pH 7.5 were collected using an Anton Paar SAXSpace instrument at the CSIR-Central Drug Research Institute (Lucknow, India) using a Dectris Mythen 2R1K detector at a sample-detector distance of 0.3 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.80 mg/ml was measured at 10°C. Two successive 1800 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Experimental molecular weight was calculated by size exclusion chromatography using Superdex-200 Increase GL 30/10 column (GE healthcare) which was prior to experiments calibrated with standard molecular weight protein markers. CAUTION! A non-linear/poorly defined Guinier region is noted (lnI(s) vs s^2 for sRg<1.3). CAUTION! Possible aggregation. CAUTION! The atomistic model (middle) does not fit the data. CAUTION! The atomistic model is missing the C-terminal histidine tag that is present in the SAXS sample construct.

Probable transcriptional regulatory protein (Probably AsnC-family) (Lrp/AsnC)
Mol. type   Protein
Organism   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Olig. state   Octamer
Mon. MW   17.9 kDa
 
UniProt   P71888 (1-148)
Sequence   FASTA