The dimeric ectodomain of the alkali-sensing insulin receptor-related receptor (ectoIRR) has a droplike shape.

Shtykova EV, Petoukhov MV, Mozhaev AA, Deyev IE, Dadinova LA, Loshkarev NA, Goryashchenko AS, Bocharov EV, Jeffries CM, Svergun DI, Batishchev OV, Petrenko AG, J Biol Chem 294(47):17790-17798 (2019) Europe PMC

SASDKS3 – The dimeric ectodomain of the alkali-sensing insulin receptor–related receptor (ectoIRR) at pH7

Insulin receptor-related protein

MW^experimental	280	kDa
MW^expected	201	kDa
V^Porod	444	nm³

log I(s) 1.63×10⁴ 1.63×10³ 1.63×10² 1.63×10¹

Insulin receptor-related protein small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Insulin receptor-related protein Guinier plot

ln 1.64×10⁴ R_g: 5.4 nm 0 (5.4 nm)^-2 s²

(sR_g)²I(s)/I(0)

Insulin receptor-related protein Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 5.4 nm 0 D_max: 19.5 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.4

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.003
1.101

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Insulin receptor-related protein CORAL model

Synchrotron SAXS data from solutions of the dimeric ectodomain of the alkali-sensing insulin receptor–related receptor (ectoIRR) in 150 mM NaCl, 20 mM Tris, pH 7 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 2M detector at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.00 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample detector distance = UNKNOWN

Insulin receptor-related protein (ectoIRR)
Mol. type		Protein
Organism		Homo sapiens
Olig. state		Dimer
Mon. MW		100.7 kDa

UniProt		P14616 (28-918)
Sequence		FASTA