HBV G-quad

Trushar Patel.

SASDKS6 – G-quadruplex DNA from Hepatitis B virus, mutant (HBV G4 G1748A + G1748A)

HBV G4 G1748A + G1748A
MWexperimental 7 kDa
MWexpected 7 kDa
VPorod 23 nm3
log I(s) 3.70×10-3 3.70×10-4 3.70×10-5 3.70×10-6
HBV G4 G1748A + G1748A small angle scattering data  s, nm-1
ln I(s)
HBV G4 G1748A + G1748A Guinier plot ln 3.70×10-3 Rg: 2.0 nm 0 (2.0 nm)-2 s2
(sRg)2I(s)/I(0)
HBV G4 G1748A + G1748A Kratky plot 1.104 0 √3 sRg
p(r)
HBV G4 G1748A + G1748A pair distance distribution function Rg: 2.0 nm 0 Dmax: 6.0 nm

Data validation

Fits and models

log I(s)
 s, nm-1
HBV G4 G1748A + G1748A DAMMIN model
log I(s)
 s, nm-1
HBV G4 G1748A + G1748A DAMMIN model
log I(s)
 s, nm-1
HBV G4 G1748A + G1748A DAMMIN model
Synchrotron SAXS data from solutions of G-quadruplex DNA from Hepatitis B virus mutant in 20 mM HEPES, 100 mM KCl, 1 mM EDTA, pH 7.5 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). 620 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Concentration: UNKNOWN.

HBV G4 G1748A + G1748A (HBV G4 G1748A + G17)
Mol. type   DNA
Organism   Hepatitis B virus
Olig. state   Monomer
Mon. MW   7.3 kDa
Sequence   FASTA