The structure of the Moco carrier protein from Rippkaea orientalis.

Krausze J, Hercher TW, Archna A, Kruse T, Acta Crystallogr F Struct Biol Commun 76(Pt 9):453-463 (2020) Europe PMC

SASDKT9 – The Moco-free form of the Moco carrier protein from Rippkaea orientalis

p450 cytochrome, putative (Moco carrier protein)
MWexperimental 62 kDa
MWexpected 75 kDa
VPorod 107 nm3
log I(s) 9.22×101 9.22×100 9.22×10-1 9.22×10-2
p450 cytochrome, putative (Moco carrier protein) small angle scattering data  s, nm-1
ln I(s)
p450 cytochrome, putative (Moco carrier protein) Guinier plot ln 9.23×101 Rg: 2.8 nm 0 (2.8 nm)-2 s2
(sRg)2I(s)/I(0)
p450 cytochrome, putative (Moco carrier protein) Kratky plot 1.104 0 √3 sRg
p(r)
p450 cytochrome, putative (Moco carrier protein) pair distance distribution function Rg: 2.7 nm 0 Dmax: 8.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
p450 cytochrome, putative (Moco carrier protein) PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of the Moco-free form of the moco carrier protein in 100 mM Tris-HCl, 300 mM NaCl, 2 %(v/v) glycerol, pH 8 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 11.50 mg/ml was measured at 4°C. 10 successive 0.500 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

p450 cytochrome, putative (Moco carrier protein) (RoMCP)
Mol. type   Protein
Organism   Rippkaea orientalis (strain PCC 8801)
Olig. state   Tetramer
Mon. MW   18.9 kDa
 
UniProt   B7K4Z0 (1-163)
Sequence   FASTA
 
PDB ID   6Y01