| MWexperimental | 29 | kDa |
| MWexpected | 75 | kDa |
| VPorod | 73 | nm3 |
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log I(s)
3.14×100
3.14×10-1
3.14×10-2
3.14×10-3
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s, nm-1
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Despite the odds: formation of the SARS-CoV-2 methylation complex
Matsuda A, Plewka J, Rawski M, Mourão A, Zajko W, Siebenmorgen T, Kresik L, Lis K, Jones A, Pachota M, Karim A, Hartman K, Nirwal S, Sonani R, Chykunova Y, Minia I, Mak P, Landthaler M, Nowotny M, Dubin G, Sattler M, Suder P, Popowicz G, Pyrć K, Czarna A, Nucleic Acids Research (2024) DOISASDKU6 – SARS-CoV-2 non-structural protein 10/non-structural protein 14 complex (nsp10/nsp14 )
Replicase polyprotein 1ab (non-structural protein 14)Replicase polyprotein 1a (non-structural protein 10)
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Fits and models
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Synchrotron SAXS data from solutions of the SARS-CoV-2 nsp10/nsp14 complex in 50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 2 mM β-mercaptoethanol, pH 8.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.1 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample at 3.4 mg/ml was injected onto a Agilent Bio SEC-3, 300 Å column. 61 successive frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
CAUTION! A significant discrepancy is noted between the experimentally determined (29 kDa) vs. expected (75 kDa) molecular weights. CAUTION! The Porod volume (73 nm^3) is inconsistent with the formation of a 75 kDa complex (expected Porod volume = 120 nm^3). Experimental temperature: UNKNOWN. X-ray exposure time: UNKNOWN. SEC-injection volume: UNKNOWN. SEC-flow rate: UNKNOWN. |
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