Structural analyses of an RNA stability element interacting with poly(A)

Torabi S, Chen Y, Zhang K, Wang J, DeGregorio S, Vaidya A, Su Z, Pabit S, Chiu W, Pollack L, Steitz J, Proceedings of the National Academy of Sciences 118(14):e2026656118 (2021) DOI

SASDKW2 – Wild type double Element for Nuclear Expression (dENE) + Poly(A)30

Wild type dENE + Poly(A)30
MWexperimental 33 kDa
MWexpected 33 kDa
log I(s) 9.15×100 9.15×10-1 9.15×10-2 9.15×10-3
Wild type dENE + Poly(A)30 small angle scattering data  s, nm-1
ln I(s)
Wild type dENE + Poly(A)30 Guinier plot ln 9.16×100 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
Wild type dENE + Poly(A)30 Kratky plot 1.104 0 √3 sRg
p(r)
Wild type dENE + Poly(A)30 pair distance distribution function Rg: 3.1 nm 0 Dmax: 10.6 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Wild type double Element for Nuclear Expression (dENE) + Poly(A)30 in 50 mM KCl, 1.0 mM MgCl2, 10 mM MOPS, 25 uM EDTA, pH 7 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA) using a Pilatus3 S 1M detector at a sample-detector distance of 3.0 m and at a wavelength of λ = 0.092 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.66 mg/ml was measured at 10°C. 15 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Wild type dENE + Poly(A)30 (WT dENE + Poly(A)30)
Mol. type   RNA
Olig. state   Monomer
Mon. MW   32.8 kDa
Sequence   FASTA