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Synchrotron SAXS data from solutions of the B. burgdorferi fibronectin-binding protein BBK32 complement inhibitory domain in 10 mM HEPES, pH 7.3, 140 mM NaCl, were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 2.1 m and at a wavelength of λ = 0.1127 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 55.00 μl sample at 8 mg/ml was injected at a 0.50 ml/min flow rate onto a Shodex KW-802.5 column at 20°C. 3 second X-ray exposures were collected continuously during the entire 30 min elution.The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted from the appropriate sample peak frames.
The beam line is equipped with an online Agilent 1260 Infinity HPLC system with a series of UV at 280 and 260 nm, multi-angle light scattering (MALS), quasi-elastic light scattering (QELS), and refractometer detectors. MALS experiments were performed using an 18-angle DAWN HELEOS II light scattering detector connected in tandem to an Optilab refractive index concentration detector (Wyatt Technology).
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s, nm-1
