pUL21 is a viral phosphatase adaptor that promotes herpes simplex virus replication and spread.

Benedyk TH, Muenzner J, Connor V, Han Y, Brown K, Wijesinghe KJ, Zhuang Y, Colaco S, Stoll GA, Tutt OS, Svobodova S, Svergun DI, Bryant NA, Deane JE, Firth AE, Jeffries CM, Crump CM, Graham SC, PLoS Pathog 17(8):e1009824 (2021) Europe PMC

SASDKW8 – Full-length pUL21 with C-terminal His-tag

Tegument protein UL21

MW^experimental	60	kDa
MW^expected	59	kDa
V^Porod	90	nm³

log I(s) 2.79×10^-2 2.79×10^-3 2.79×10^-4 2.79×10^-5

Tegument protein UL21 small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

ln 2.79×10^-2 R_g: 4.2 nm 0 (4.2 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

p(r)	R_g: 4.5 nm 0 D_max: 18.1 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.5

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.222
1.011

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Full-length pUL21 with C-terminal His-tag Rg histogram

R_g, nm

Synchrotron SAXS data from solutions of full-length pUL21 in 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, were collected on the EMBL-P12 bioSAXS beam line at Petra III (DESY Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.1 and 1.7 mg/ml were measured at 20°C. 47 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

The reported experimental SAS data were obtained by averaging data sets measured from two dilutions: 1.11 mg/ml and 1.66 mg/ml.

Tegument protein UL21
Mol. type		Protein
Organism		Human alphaherpesvirus 1 strain KOS
Olig. state		Monomer
Mon. MW		58.7 kDa

UniProt		P10205 (1-535)
Sequence		FASTA