| MWI(0) | 104 | kDa |
| MWexpected | 112 | kDa |
| VPorod | 165 | nm3 |
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log I(s)
2.97×104
2.97×103
2.97×102
2.97×101
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s, nm-1
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The USR domain of USF1 mediates NF-Y interactions and cooperative DNA binding
Bernardini A, Lorenzo M, Chaves-Sanjuan A, Swuec P, Pigni M, Saad D, Konarev P, Graewert M, Valentini E, Svergun D, Nardini M, Mantovani R, Gnesutta N, International Journal of Biological Macromolecules (2021) DOISASDKW9 – Transcription factors USF1 and NF-Y bound to a CCAAT DNA box(50bp)
Upstream stimulatory factor 1Nuclear transcription factor Y subunit alpha
Nuclear transcription factor Y subunit beta
Nuclear transcription factor Y subunit gamma
DNA 50bp
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Fits and models
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Synchrotron SAXS data from solutions of transcription factors USF1 and NF-Y bound to a 50 base pair CCAAT DNA box in 100 mM cacodylate buffer, pH 6.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 15°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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