Spatial Organization of Dps and DNA-Dps Complexes.

Dubrovin EV, Dadinova LA, Petoukhov MV, Yu Soshinskaya E, Mozhaev AA, Klinov DV, Schäffer TE, Shtykova EV, Batishchev OV, J Mol Biol :166930 (2021) Europe PMC

SASDKY4 – DNA binding protein of starvation (no EDTA)

DNA protection during starvation protein
MWexperimental 192 kDa
MWexpected 225 kDa
VPorod 277 nm3
log I(s) 2.46×103 2.46×102 2.46×101 2.46×100
DNA protection during starvation protein small angle scattering data  s, nm-1
ln I(s)
DNA protection during starvation protein Guinier plot ln 2.46×103 Rg: 3.8 nm 0 (3.8 nm)-2 s2
(sRg)2I(s)/I(0)
DNA protection during starvation protein Kratky plot 1.104 0 √3 sRg
p(r)
DNA protection during starvation protein pair distance distribution function Rg: 3.7 nm 0 Dmax: 11.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
DNA protection during starvation protein DAMMIN model
log I(s)
 s, nm-1
DNA protection during starvation protein CORAL model
Synchrotron SAXS data from solutions of DNA binding protein of starvation in 20мМ Tris-HCl, 100 мМ NaCl, pH 7.5 were collected on the EMBL P12 beam line at PETRA III (DESY, Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.50 mg/ml was measured at 20°C. 29 successive 0.100 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

DNA protection during starvation protein (DPS)
Mol. type   Protein
Organism   Escherichia coli
Olig. state   Dodecamer
Mon. MW   18.7 kDa
 
UniProt   A7ZJM7 (1-167)
Sequence   FASTA