| MWI(0) | 186 | kDa |
| MWexpected | 171 | kDa |
| VPorod | 354 | nm3 |
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log I(s)
5.89×101
5.89×100
5.89×10-1
5.89×10-2
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s, nm-1
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Structural features of a minimal intact methyltransferase of a type I restriction-modification system.
Seo PW, Hofmann A, Kim JH, Hwangbo SA, Kim JH, Kim JW, Huynh TYL, Choy HE, Kim SJ, Lee J, Lee JO, Jin KS, Park SY, Kim JS, Int J Biol Macromol (2022) Europe PMCSASDKY9 – A minimal intact methyltransferase of a type I restriction-modification system
Type I restriction-modification system methyltransferase subunitProtein Ocr
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Fits and models
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Synchrotron SAXS
data from solutions of
A minimal intact methyltransferase of a type I restriction-modification system
in
20 mM Tris-HCl,150 mM NaCl, pH 7.5
were collected
on the
4C beam line
at the Pohang Accelerator Laboratory storage ring
(Pohang, South Korea)
using a Rayonix SX165 detector
at a sample-detector distance of 4 m and
at a wavelength of λ = 0.0734 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 100.00 μl sample
at 7 mg/ml was injected at a 0.06 ml/min flow rate
onto a Agilent Bio SEC-5, 300Å column
at 25°C.
One
10 second frame was collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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