RNA triplex structures revealed by WAXS-driven MD simulations

Chen Y, He W, Kirmizialtin S, Pollack L, (2022) DOI

SASDKZ5 – RNA Long Tetraloop Hairpin Triplex in 200 mM KCl

RNA Long Tetraloop Hairpin Triplex
MWexperimental 28 kDa
MWexpected 28 kDa
log I(s) 9.60×101 9.60×100 9.60×10-1 9.60×10-2
RNA Long Tetraloop Hairpin Triplex small angle scattering data  s, nm-1
ln I(s)
RNA Long Tetraloop Hairpin Triplex Guinier plot ln 9.61×101 Rg: 2.8 nm 0 (2.8 nm)-2 s2
(sRg)2I(s)/I(0)
RNA Long Tetraloop Hairpin Triplex Kratky plot 1.104 0 √3 sRg
p(r)
RNA Long Tetraloop Hairpin Triplex pair distance distribution function Rg: 2.8 nm 0 Dmax: 10.3 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of RNA Long Tetraloop Hairpin Triplex in 200 mM KCl in 200 mM KCl, 20 mM Na-MES, 50 μM EDTA, pH 5.5 were collected on the 16-ID (LiX) beam line at the National Synchrotron Light Source II (NSLS-II) storage ring (Upton, NY, USA) using a Pilatus3 S 1M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.092 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 3.15 mg/ml was measured at 10°C. 15 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

RNA Long Tetraloop Hairpin Triplex (LT-TPX)
Mol. type   RNA
Olig. state   Monomer
Mon. MW   27.9 kDa
Sequence   FASTA