| MWexperimental | 83 | kDa |
| MWexpected | 122 | kDa |
| VPorod | 245 | nm3 |
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log I(s)
3.99×103
3.99×102
3.99×101
3.99×100
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s, nm-1
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Cooperation between intrinsically disordered and ordered regions of Spt6 regulates nucleosome and Pol II CTD binding, and nucleosome assembly.
Kasiliauskaite A, Kubicek K, Klumpler T, Zanova M, Zapletal D, Koutna E, Novacek J, Stefl R, Nucleic Acids Res (2022) Europe PMCSASDL22 – Transcription elongation factor SPT6 - ΔtSH2 variant
Transcription elongation factor SPT6 - ΔtSH2 variant|
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Fits and models
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Rg, nm
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Synchrotron SAXS
data from solutions of
Transcription elongation factor SPT6 - ΔtSH2 variant
in
25 mM NaPi; 150mM NaCl; 0.5 mM EDTA; 5% glycerol; 1 mM DTT, pH 7.5
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3 m and
at a wavelength of λ = 1.24 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 5.00 mg/ml was measured
at 15°C.
20 successive
0.045 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
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