| MWexperimental | 36 | kDa |
| MWexpected | 34 | kDa |
| VPorod | 41 | nm3 |
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log I(s)
2.44×102
2.44×101
2.44×100
2.44×10-1
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s, nm-1
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Fine-tuning of intrinsic N-Oct-3 POU domain allostery by regulatory DNA targets
Alazard R, Mourey L, Ebel C, Konarev P, Petoukhov M, Svergun D, Erard M, Nucleic Acids Research 35(13):4420-4432 (2007) DOISASDL27 – N-Oct-3 POU transcription factor domain in complex with rat CRH DNA
POU domain, class 3, transcription factor 2Rat CRH DNA
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Fits and models
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Synchrotron SAXS
data from solutions of
N-Oct-3 POU transcription factor domain in complex with rat CRH DNA
in
50 mM Tris, 0.4 M NaCl, 2% glycerol, 2 mM DTT, pH 7.5
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a 1D Gas detector detector
at a sample-detector distance of 2.3 m and
at a wavelength of λ = 0.15 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 3.1 and 3.1 mg/ml were measured
at 12°C.
15 successive
60 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Storage temperature = UNKNOWN
Tags:
X33
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