Shape-function of a novel metapyrocatechase, RW4-MPC: Metagenomics to SAXS data based insight into deciphering regulators of function.

Vasudeva G, Sidhu C, Kalidas N, Ashish, Pinnaka AK, Int J Biol Macromol 188:1012-1024 (2021) Europe PMC

SASDL29 – SAXS data from RW4-MPC at 1 mg/ml at 283 K

Metapyrocatechase
MWexperimental 62 kDa
MWexpected 139 kDa
log I(s) 9.40×104 9.40×103 9.40×102 9.40×101
Metapyrocatechase small angle scattering data  s, nm-1
ln I(s)
Metapyrocatechase Guinier plot ln 9.40×104 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
Metapyrocatechase Kratky plot 1.104 0 √3 sRg
p(r)
Metapyrocatechase pair distance distribution function Rg: 3.3 nm 0 Dmax: 10.2 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Metapyrocatechase MODELLER model
log I(s)
 s, nm-1
Metapyrocatechase SREFLEX model
log I(s)
 s, nm-1
Metapyrocatechase GASBOR model
SAXS data from solutions of SAXS data from RW4-MPC at 1 mg/ml at 283 K in 20 mM Tris-HCl 250 mM NaCl, pH 8.0, were collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) using a Mythen 1K detector at a sample-detector distance of 0.3 m and at a wavelength of λ = 0.15414 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 10°C. One 3600 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

SAXS data was collected from freshly purified protein. Publication includes additional results and discussion on temperature and pH variation.

Metapyrocatechase (RW4-PC)
Mol. type   Protein
Organism   Novosphingobium sp. AAP93
Olig. state   Tetramer
Mon. MW   34.6 kDa
 
UniProt   A0A0N1BQH2 (1-311)
Sequence   FASTA
 
PDB ID   1F1V