Structural, thermodynamic and functional studies of human 71 kDa heat shock cognate protein (HSPA8/hHsc70)

Silva N, de Camargo Rodrigues L, Dores-Silva P, Montanari C, Ramos C, Barbosa L, Borges J, Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics :140719 (2021) DOI

SASDL42 – Human Heat Shock Cognate 71 kDa Protein (HSPA8 / Hsc70)

Heat shock cognate 71 kDa protein
MWexperimental 72 kDa
MWexpected 71 kDa
VPorod 111 nm3
log I(s) 1.13×106 1.13×105 1.13×104 1.13×103
Heat shock cognate 71 kDa protein small angle scattering data  s, nm-1
ln I(s)
Heat shock cognate 71 kDa protein Guinier plot ln 1.13×106 Rg: 3.7 nm 0 (3.7 nm)-2 s2
(sRg)2I(s)/I(0)
Heat shock cognate 71 kDa protein Kratky plot 1.104 0 √3 sRg
p(r)
Heat shock cognate 71 kDa protein pair distance distribution function Rg: 3.5 nm 0 Dmax: 11.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Human Heat Shock Cognate 71 kDa Protein (HSPA8 / Hsc70) Rg histogram Rg, nm
Heat shock cognate 71 kDa protein EOM/RANCH model
Heat shock cognate 71 kDa protein EOM/RANCH model
Heat shock cognate 71 kDa protein EOM/RANCH model
Heat shock cognate 71 kDa protein EOM/RANCH model
Heat shock cognate 71 kDa protein EOM/RANCH model
log I(s)
 s, nm-1
Heat shock cognate 71 kDa protein DAMMIN model
Synchrotron SAXS data from solutions of Human Heat Shock Cognate 71 kDa Protein (HSPA8 / Hsc70) in 25 mM Tris HCl, 50 mM NaCl, 5 mM Sodium Phosphate, 5 mM KCl, pH 7.5 were collected on the SAXS1 Beamline beam line at the Brazilian Synchrotron Light Laboratory storage ring (Campinas, Brazil) using a Pilatus 300K detector at a sample-detector distance of 1 m and at a wavelength of λ = 0.1488 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 1.00 mg/ml was measured at 27°C. 10 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN

Heat shock cognate 71 kDa protein (HSPA8, Hsc70)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   70.9 kDa
 
UniProt   P11142 (1-646)
Sequence   FASTA