| MWexperimental | 176 | kDa |
| MWexpected | 108 | kDa |
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log I(s)
8.49×10-2
8.49×10-3
8.49×10-4
8.49×10-5
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s, nm-1
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A structural study of the cytoplasmic chaperone effect of 14-3-3 proteins on Ataxin-1.
Leysen S, Jane Burnley R, Rodriguez E, Milroy LG, Soini L, Adamski CJ, Nitschke L, Davis R, Obsil T, Brunsveld L, Crabbe T, Yahya Zoghbi H, Ottmann C, Martin Davis J, J Mol Biol :167174 (2021) Europe PMCSASDL46 – 14-3-3zeta AXH-C complex
14-3-3 protein zeta/deltaAtaxin-1 AXH-C
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Fits and models
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Synchrotron SAXS
data from solutions of
14-3-3zeta AXH-C complex
in
20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH 7.5
were collected
on the
B21 beam line
at the Diamond Light Source storage ring
(Didcot, UK)
using a Pilatus 2M detector
at a sample-detector distance of 4.0 m and
at a wavelength of λ = 0.099987 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample
at 11 mg/ml was injected
onto a Shodex KW400 series column
at 25°C.
620 successive
3 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Storage temperature = UNKNOWN. Flow rate = UNKNOWN |
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